Survey the effect of inulin and green tea extract on survival of Bifidobacterium longum and physicochemical properties of synbiotic soybean ice cream

Document Type : Complete scientific research article

Author

Assistant professor, Department of Food Science and Technology, Faculty of Agriculture, Varamin-Pishva Branch, Islamic Azad University, Varamin, Iran

Abstract

Abstract
Background and objectives: Today, consumers tend to eat fresh food with high nutritional and health benefits. Food, in addition to eliminating hunger and providing essential nutrients, should improve the health and physical and mental health of the consumer. Probiotics are living microorganisms that have a healthy effect if they are consumed alive and sufficiently. Using appropriate prebiotic compounds and selecting suitable carriers for probiotics can increase the growth and activity of these beneficial bacteria. Soybeans and their products are considered to be good carriers for probiotic microorganisms that can play an effective role in increasing the viability of probiotic bacteria. The purpose of this study was to investigate the effects of inulin prebiotic and antioxidant green tea on increasing the viability time of Bifidobacterium longum in synbiotic soy ice cream.
Materials and methods: In order to produce synbiotic soy ice cream, the green tea extract in concentrations of 1, 2 and 3% and inulin in proportions of 1, 2 and 3% and also probiotic bacteria Bifidobacterium Longum in the amount of 107 Cfu/ml were added to synbiotic soy ice cream formulation. Therefore, 10 treatments were designed according to completely randomized design with three replicates. Tests of determination the percentage of polyphenolic compounds, percentage of total acidity, percentage of increasing overrun, peroxide number (mEq/Kg), viscosity evaluation (cp), color assessment (lighting, redness and yellowness index), sensory assessments (odor, taste, texture and overall acceptance) were performed one day after production and the survival rate of Bifidobacterium Longum (Cfu/ml) of soybean ice cream samples done during 21 days of storage at -18°C. For data analysis of the results of the tests, Duncan's test for comparison means at probability level of 5% was used by Minitab version 16 software.
Results: The results showed that increasing the concentration of inulin and green tea extract in the synbiotic soy ice cream formulation, overrun, yellowness index (b*), lighting index (L*), score of sensory indexes including odor, taste, texture and overall acceptance decreased and acidity, the viability rate of Bifidobacterium longum, viscosity and redness index (a*) increased. Bifidobacterium longum population after 21 days of storage in all treatments, including control, was higher than 106 Cfu/ml. In contrast to inulin increase in the concentration of green tea extract had a significant effect on the reduction of peroxide index. The sensory indices of the treatment containing 1% green tea extract and inulin were not significantly different with the control sample and were presented as superior treatment in this study. Sensory indexes of treatments containing 1% green tea extract and inulin were not significantly different with the control sample and were presented as a superior treatment in this study.
Conclusion: By using 1% green tea extract and 1% inulin can be possible to produce a synbiotic soy ice cream with optimal qualitative and sensory properties with good health properties.

Keywords


  1. Akalin, A., and Erişir, D. 2008. Effects of inulin and oligofructose on the rheological characteristics and probiotic culture survival in low-fat probiotic ice cream. J. of Food Science. 73: 4.184-188.
  2. Akalin, A.S., Karagözlü, C., and Ünal, G. 2008. Rheological properties of reduced-fat and low-fat ice cream containing whey protein isolate and inulin. European Food Research and Technology. 227: 2.889-895.
  3. Akbari, M., Eskandari, M.H., and Niakosari, M. 2016. The effect of inulin on the physicochemical properties and sensory attributes of low-fat ice cream. International Dairy J. 57: 52-55.
  4. Baruah, S., Bordolor, A.K., Gogor, M.K. Gogoin, R.C., and Hazarika, M. 2012. Study of antioxidant property in different types of tea and its utilization in the development of some popular items like tea ice cream. Two and a bud. 59: 2.102-105.
  5. Bhatnagar, S., and Aggarwal, R. 2007. Lactose intolerance. BMJ. 334: 7608.1331­1332.
  6. Chen, Zh. U., Wang, L. Y., Chan, P.T., Zhang, Z., Chung, H. Y., and Liang, C. 1998. Antioxidant activity of green tea catechin extract compared with that of rosemary extract. J. of the American Oil Chemists' Society. 75: 9.1141-1145.
  7. De Lacey, A.L., Pérez-Santín, E., López-Caballero, M., and Montero, P. 2014. Survival and metabolic activity of probiotic bacteria in green tea. LWT-Food Science and Technology. 55: 1.314-322.
  8. Golestani, M., Pour Ahmad, R., and Mahdavi Adeli, H.R. 2016. Effect of inulin on the survival of probiotic bacteria, physical, chemical and sensory properties of fermentation and non-fermented synbiotic ice cream. Food technology and nutrition. 3: 3.25-32. (In Persian).
  9. Ghaderi, S., Mazaheri Tehrani, M., and Razavi, S.M. 2016. An investigation on the effect of aging time on the physicochemical, rheological and textural properties of vegetable-based ice cream of soy and sesame. Iranian Food Science and Technology Research J. 13: 4.528-539. (In Persian).
  10. Guarner, F. 2005. Inulin and oligofructose: Impact on intestinal diseases and disorders. British J. of Nutrition. 93: 1.61-65.
  11. Fathi-Achachlouei, B., and Mahmoudi Moghas, H. 2018. Effect of different levels of Inulin andchitosan on the physicochemical, sensory properties and survival of probiotics in synbiotic Doogh. J. of Food Science and Technology. 75: 15.235-245. (In Persian).
  12. Hashemi, M., Gheisari, H.R., and Shekarforoush, S.S. 2015. Evaluation of physicochemical, textural and sensorial characteristics of low-fat or low-sugar synbiotic ice-cream. J. of Food Hygiene. 5: 18. 71-103.
  13. Homayouni Rad, A., Javadi, M., Ghaseminejad Tabrizian, V., and Alizadeh M. 2012. Increasing the probiotic survival in functional ice cream by microencapsulation. J. of Food industry research. 22: 1.91-97. (In Persian).
  14. Homayouni Rad, A., Norouzi, Sh., and Asghari Jafarabadi, M. 2014. Effects of soymilk powder on physicochemical, microbial and sensory properties of fermented symbiotic soy ice-cream. J. of Food Research. 24: 1.19-30. (In Persian).
  15. Hosseininezhad, M., Anvari, H., Zhiani, M., and Abedfar, A. 2017. Evaluation the effect of inulin supplementary on the sensory and textural properties of probiotic bread (Taftoon). J. of Innovation in Food Science and Technology. 6: 2.185-198. (In Persian).
  16. Institute of Standards and Industrial Research of Iran. 1997. Characteristics and method of ice cream test. No. 2450 (In Persian).
  17. Institute of Standards and Industrial Research of Iran. 1998. Oils and edible fats- measuring peroxide value. No. 2450 (In Persian).
  18. Jaziri, I.N., Slama, M.B., Mhadhbi, H., Urdaci, M.C., and Hamdi, M. 2009. Effect of green and black teas (Camellia sinensis L.) on the characteristic microflora of yogurt during fermentation and refrigerated storage. Food Chemistry. 112: 3.614-620.
  19. Kavaz Yüksel, K., Yüksel, M., and Güngör Sat, I. 2017. Determination of certain physicochemical characteristics and sensory properties of green tea powder (matcha) added ice creams and detection of their organic acid and mineral contents. GIDA. 42: 2.116-126.
  20. López de Dicastillo, C., Del Mar Castro-López, M., ManuelLópez-Vilariño, J., and Victoria González-Rodríguez, M. 2008. Immobilization of green tea extract on polypropylene films to control the antioxidant activity in food packaging. Food Research International. 53: 1.522–528.
  21. Lum, A. K., and Albrecht, J. A. 2008. Sensory evaluation of ice cream made with prebiotic ingredients, Rurals: Review of undergraduate research in agricultural and life sciences. 3: 1.1-9
  22. Mahdian, E., Mazaheri Tehrani, M., and Shahidi, F. 2011. Evaluation of the effect of soy flour on rheological properties of ice cream. Iranian J. of Food Science and Technolog. 8: 31.107-114. (In Persian).
  23. Mahdian, E., Karajiyan, R., and Sabri, S. 2013. Evaluation of the effect of replenishment of milk fat with inulin and milk protein concentrate on physico-chemical and sensory properties of low-fat ice cream. Innovation in Food Science and Technology. 5: 4.43-51. (In Persian).
  24. Maleknejad, A.R., Nateghi, L., and Shahab Lavasani, A. R. 2018. Effects of adding green tea powder on physicochemical, sensory and antioxidant properties of ice cream. J. of Food Science and Technology. 76: 15.257-268. (In Persian).
  25. Matan, N., Puangjinda, K., Phothisuwan, S., and Nisoa, M. 2014. Combined antibacterial activity of green tea extract with atmospheric radio-frequency plasma against pathogens on fresh-cut dragon fruit. Food Control. 50: 291-296.
  26. Mostafavi, F., Mazaheri Tehrani, M., and Mohebbi, M. 2014. Effect of fat content on rheological and physical properties of vanilla ice cream. J. of Food Science and Technology. 11: 45. 55-64. (In Persian).
  27. Molan, A.L., De, S., and Meagher, L. 2008. Antioxidant activity and polyphenol content of green tea flavan-3-ols and oligomeric pro anthocyanidins. International J. of Food Sciences and Nutrition. 60: 6.497-506.
  28. Mortazavi Nejad, S., Abbasi, H., and Jahadi, M. 2015. Optimization of sponge cake formulation containing okara. J. of Research and Innovation in Food Science and Technology. 5: 1.1-14. (In Persian).
  29. Naeemi, H., Mortazavi, S. A., Milani, E., and Koochaki, A. 2013. The influence of adding Inulin and encapsulation on survivability lactobacillus casei storage of synbiotic yoghurt. J. of Food Science and Technology. 10: 40.27-36. (In Persian).
  30. Nahardani, M., Hosseini Nejad, M., and Elhami Rad, A.H. 2012. Evaluation of prebiotic effects and qualitative properties of inulin extracted from Non-indigenous Chicory of
    Iran. J. of Innovation in Food Science and Technology. 4: 4.87-96. (In Persian).
  31. Namal Senanayake, S.P.J. 2013. Green tea extract: Chemistry, antioxidant properties and food applications – A review. J. of Functional Foods. 5: 4.1529-1541.
  32. Nasiri Rad, R., Haddad Khodaparast, M.H., Elhami Rad, A.H., and Rufigari Haghighat, Sh. 2012. Effect of harvest season and breeding conditions on the total amount of phenolic compounds of Iranian green tea. Iranian J. of Food Science and Technology. 8: 4.349-352. (In Persian).
  33. Nazari, B., Haghnazari, S., and Bolandi, M. 2015. Formulation and preparation of ice cream replacing sugar with sucralose and its organoleptic characteristics. J. of Food Science and Technology. 49: 12. 145-153. (In Persian).
  34. Nick Joe, R., Nourian, N., and Aziz Nia, S. 2013. A review of the antioxidant properties of green tea. 21st National Congress of Food Science and Technology. Shiraz University. Shiraz (In Persian).
  35. Noori, N., Noudoost, B., Gandomi Nasrabadi, H., and Akhondzadeh Basti, A. 2017. Effects of green tea extract nano encapsulation on the survival of Lactobacillus casei and Bifidobacterium lactis in symbiotic ice cream. J. of Veterinary Research. 72: 2.195-205.
  36. Pandiyan, C., Annal Villi, R., Kumaresan, G. Murugan, B., and Rajarajan, G. 2012. Effect of incorporation of inulin on the survivability of Lactobacillus acidophilus in symbiotic ice cream. International Food Research J. 19: 4.1729-1732.
  37. Pintor, A., Escalona-Buendia, H.B., and Totosaus, A. 2017. Effect of inulin on melting and textural properties of low-fat and sugar reduced ice cream: Optimization via a response surface methodology. International Food Research J. 24: 4.1728-1734.
  38. Rahimi Tabalvandani, S., Abbasi, S., and Azizi Tabrizzad, M. H. 2008. Production of operator chewing with using citric acid, encapsulated with inulin and casein. 18th National Congress of Food Science and Technology. Khorasan Razavi Research Institute of Food Science and Technology. Mashhad (In Persian).
  39. Ranjbar Nadamani, A., Sadeghi Mahounak, A.R., Ghorbani, M., and Kashaninejad, M. 2014. Antioxidant interaction analysis of the combination of green tea and rosemary extracts. Iranian Food Science and Technology Research J. 10: 3. 232-240. (In Persian).
  40. Rashidi Nejad, A., John Bircha, E., Sun-Waterhousec, D., and Everett, D.W. 2014. Delivery of green tea catechin and epigallocatechin gallate in liposomes incorporated into low-fat hard cheese. Food Chemistry. 156: 176–183.
  41. Seyyedi, Sh., Hashemi Ravan, M., and Nourbakhsh, F. 2012. Growth of Lactobacillus acidophilus and Bifidobacterium lactis in synbiotic orange juice. 2th National Food Security Seminar. Savadkou Islamic Azad University. Savadkou (In Persian).
  42. Tamminen, M., Salminen, S.J., and Ouwehand A. 2013. Fermentation of carrot juice by synbiotic: Viability and preservation of adhesion. International J. of Biotechnology for Wellness Industries. 2:10-15.
  43. Zhao, D., and Shah, N.P. 2014. Influence of tea extracts supplementation on Bifidobacteria during soymilk fermentation. International J. of Food Microbiology.188: 36–44.
  44. Wardani, S., Niwat, C., and Ahza, A.B. 2016. Quality performance of Indonesian frozen dessert (es puter) enriched with black glutinous rice (Oryza sativa glutinosa L.) and green tea (Camellia sinensis L). International Food Research J. 23: 2.482- 490.