Evaluation and comparison of functional properties of whey protein isolate-inulin mixtures and conjugates

Document Type : Complete scientific research article

Authors

1 PhD student, Faculty of Food Science and Technology, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran

2 Faculty of Food Science and Technology, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran

Abstract

Evaluation and comparison of functional properties of whey protein isolate-inulin mixtures and conjugates

Abstract
Background and objectives:Proteins are sensitive to environmental stresses, which limits their nutritional applications. Millard reaction leads to increases the functional and biological properties of proteins.Whey protein isolate has a wide range of applications in the food industry, but is sensitive to to the certain environmental conditions and its functional properties are reduced. Inulin is a water-soluble polysaccharide widely used in the food and pharmaceutical industries.The aim of the present study was to prepare and compare the mixture and conjugate of whey protein isolate-inulin, different weight ratios of protein-polysaccharide (1:1, 1:2 and 2:1) and investigate their functional properties.

Materials and methods:Glycation via Maillard reaction (MR) done at 60 ◦C and a relative humidity of 79%, during 24 h. In this regard, polyacrylamide gel electrophoresis (SDS-PAGE), Fourier transform infrared spectroscopy (FT-IR) was performed to confirm the Millard reaction, and also evaluated DPPH free radical scavenging activity, (DSC), emulsifying activity index and emulsion stability index as well as viscosity.

Results: The results of SDS-PAGE patterns and FT-IR validated the occurrence of the Maillard reaction between whey protein isolate and inulin. Also, the results indicated that the whey protein isolate and inulin conjugates have better functional properties than natural proteins and mixture of the whey protein isolate and inulin. Whey protein isolate - inulin conjugates at ratio of 2:1 showed higher emulsifying activity index, emulsion stability index and antioxidant activity, whereas the emulsions formed by conjugated whey protein isolate - inulin at ratio of 1:2 showed the highest viscosity.



Conclusion: Whey protein isolate glycation via Maillard reaction improve the thermal stability, antioxidant activity and emulsion stability of samples. The Maillard reaction is an effective process of improving the functional properties of proteins, so It causes the widespread use of whey protein isolate in food industry. The Maillard reaction conjugates of whey protein isolate and inulin have been used in food products such as mayonnaise and salads dressing, dairy products and diet products.
Conclusion: Whey protein isolate glycation via Maillard reaction improve the thermal stability, antioxidant activity and emulsion stability of samples. The Maillard reaction is an effective process of improving the functional properties of proteins, so It causes the widespread use of whey protein isolate in food industry. The Maillard reaction conjugates of whey protein isolate and inulin have been used in food products such as mayonnaise and salads dressing, dairy products and diet products.

Keywords


  1. Qi, P.X., Xiao, Y. and Wickham, E.D. 2017. Stabilization of whey protein isolate (WPI) through interactions with sugar beet pectin (SBP) induced by controlled dry-heating. Food Hydrocolloids. 67:1-13.
  2. Chen, W., Lv, R., Wang, W., Ma, X., Muhammad, A.I., Guo, M., Ye, X. and Liu, D. 2019. Time effect on structural and functional properties of whey protein isolate‐gum acacia conjugates prepared via Maillard reaction. Journal of the Science of Food and Agriculture. 99: 4801-4807.
  3. De Oliveira, F.C., Coimbra, J.S.D.R., de Oliveira, E.B., Zuñiga, A.D.G., and Rojas, E.E.G. 2016. Food protein-polysaccharide conjugates obtained via the Maillard reaction: A review. Critical Reviews in Food Science and Nutrition. 56: 1108-1125.
  4. Li, M., McClements, D.J., Liu, X., and Liu, F. 2020. Design principles of oil‐in‐water emulsions with functionalized interfaces: Mixed, multilayer, and covalent complex structures. Comprehensive Reviews in Food Science and Food Safety. 19: 3159-3190.
  5. Dong, X., Du, S., Deng, Q., Tang, H., Yang, C., Wei, F., Chen, H., Quek, S.Y., Zhou, A. and Liu, L. 2020. Study on the antioxidant activity and emulsifying properties of flaxseed gum-whey protein isolate conjugates prepared by Maillard reaction. International Journal of biological macromolecules. 153:1157-1164.
  6. Nooshkam, M., and Varidi, M. 2020. Maillard conjugate-based delivery systems for the encapsulation, protection, and controlled release of nutraceuticals and food bioactive ingredients: A review. Food Hydrocolloids. 100:105389.
  7. Shoaib, M., Shehzad, A., Omar, M., Rakha, A., Raza, H., Sharif, H.R., Shakeel, A., Ansari, A. and Niazi, S. 2016. Inulin: Properties, health benefits and food applications. Carbohydrate polymers. 147:444-454.
  8. Morris, C. and Morris, G.A. 2012. The effect of inulin and fructo-oligosaccharide supplementation on the textural, rheological and sensory properties of bread and their role in weight management: A review. Food chemistry. 133: 237-248.
  9. Ghasab-nezhad, M., Hojjati, M., and Jooyandeh, H. 2019. Effect of inulin on physico-chemical, microbial and sensory properties of the kefir produced of buffalo milk. Journal of Food Science and Technology. 89:357-367.

10.Bitaraf, M.S., Khodaiyan, F., and Hosseini, S.S. 2018. Rheological properties of probiotic non-fat yogurt containing Lactobacillus reuteri: Effects of inulin addition, inoculum level and fermentation temperature. Journal of Food and Bioprocess Engineering. 1: 109-116.

11.Sadeghi, S., Madadlou, A., and Yarmand, M. 2014. Microemulsification –cold gelation of whey proteins for nanoencapsulation of date palm pit extract. Food Hydrocolloids. 35: 90-596.

12.Pirestani, S., Nasirpour, A., Keramat, J., Desobry, S. and Jasniewski, J. 2018. Structural properties of canola protein isolate-gum Arabic Maillard conjugate in an aqueous model system. Food Hydrocolloids. 79: 228-234.

13.Laemmli, Ulrich K. 1970. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 227: 680-685.

14.Nooshkam, M. and Madadlou, A. 2016. Maillard conjugation of lactulose with potentially bioactive peptides. Food Chemistry. 192:831-836.

15.Schmidt, U.S., Pietsch, V.L., Rentschler, C., Kurz, T., Endreß, H.U., and Schuchmann, H.P. 2015. Influence of the degree of esterification on the emulsifying performance of conjugates formed between whey protein isolate and citrus pectin. Food Hydrocolloids. 56:1-8.

16.Chen, F., Liang, L., Zhang, Z., Deng, Z., Decker, E.A., and McClements, D.J. 2016. Inhibition of lipid oxidation in nanoemulsions and filled microgels fortified with omega-3 fatty acids using casein as a natural antioxidant. Food Hydrocolloids. 63:240-248.

17.De Castro, R.J.S., Domingues, M.A.F., Ohara, A., Okuro, P.K., dos Santos, J.G., Brexó, R.P., and Sato, H.H. 2017. Whey protein as a key component in food systems: Physicochemical properties, production technologies and applications. Food structure. 14:17-29.

18.Perrechil, F.A., Santana, R.C., Lima, D. B., Polastro, M.Z., and Cunha. R.L. 2014. Emulsifying properties of maillard conjugates produced from sodium caseinate and locust bean gum. Brazilian Journal of Chemical Engineering. 31:429 - 438.

19.Mao, L., Boiteux, L., Roos, Y.H., and Miao, S. 2014. Evaluation of volatile characteristics in whey protein isolate–pectin mixed layer emulsions under different environmental conditions. Food Hydrocolloids. 41:79-85.

20.Gómez-Ordóñez, E., and Rupérez, P. 2011. FTIR-ATR spectroscopy as a tool for polysaccharide identification in edible brown and red seaweeds. Food hydrocolloids. 25:1514-1520.

21.Zhu, X., Jia, C., Meng, X., Xing, M., Yi, Y., and Gao, X. 2018. Synthesis, characterization of inulin propionate ester, and evaluation of its in vitro effect on SCFA production. Starch‐Stärke, 70:1800037.

22.Ozel, B., Aydin, O., and Oztop, M.H. 2020. In vitro digestion of polysaccharide including whey protein isolate hydrogels. Carbohydrate polymers. 229:115469.

23.Ye, M.P., Zhou, R., Shi, Y.R., Chen, H.C., and Du, Y. 2017. Effects of heating on the secondary structure of proteins in milk powders using mid-infrared spectroscopy. Journal of Dairy Science. 100: 89-95.

24.Xu, D., Wang, X., Jiang, J., Yuan, F., and Gao, Y. 2012. Impact of whey protein–Beet pectin conjugation on the physicochemical stability of β-carotene emulsions. Food Hydrocolloids. 28: 258-266.

25.González-Martínez, D.A., Carrillo-Navas, H., Barrera-Díaz, C.E., Martínez-Vargas, S.L., Alvarez-Ramírez, J., and Pérez-Alonso, C. 2017. Characterization of a novel complex coacervate based on whey protein isolate-tamarind seed mucilage. Food hydrocolloids. 72:115-126.

26.Shi, Y., Liang, R., Chen, L., Liu, H., Goff, H. D., Ma, J., and Zhong, F. 2019. The antioxidant mechanism of Maillard reaction products in oil-in-water emulsion system. Food Hydrocolloids. 87:5 82-592.

27.Apolinário, A.C., de Carvalho, E.M., de Lima Damasceno, B.P.G., da Silva, P.C.D., Converti, A., Pessoa Jr, A., and da Silva, J.A. 2017. Extraction, isolation and characterization of inulin from Agave sisalana boles. Industrial Crops and Products.108:355-362.

28.Nooshkam, M., Varidi, M., and Verma, D.K. 2020. Functional and biological properties of Maillard conjugates and their potential application in medical and food: A review. Food Research International. 131:109003.

29.Tan, S., Zhong, C., and Langrish, T. 2020. Pre-gelation assisted spray drying of whey protein isolates (WPI) for microencapsulation and controlled release. LWT. 117:108625.

30.Barth, A. 2007. Infrared spectroscopy of proteins. Biochimica et Biophysica Acta (BBA)-Bioenergetics.1767: 1073-1101.

31.Kim, J.S., and Lee, Y.S. 2009. Study of Maillard reaction products derived from aqueous model systems with different peptide chain lengths. Food Chemistry.116: 846–853.

32.Lertittikul, W., Benjakul, S., and Tanaka, M. 2007. Characteristics and antioxidative activity of Maillard reaction products from a porcine plasma protein–glucose model system as influenced by pH. Food Chemistry. 100: 669-677.

33.Gu, F.L., Kim, J.M., Abbas, S., Zhang, X.M., Xia, S.Q., and Chen, Z.X. 2010. Structure and antioxidant activity of high molecularweight Maillard reaction products from casein-glucose. Food Chemistry.120:505–511.

34.Jiang, Z., and Brodkorb, A. 2012. Structure and antioxidant activity of Maillard reaction products from α-lactalbumin and β-lactoglobulin with ribose in an aqueous model system. Food Chemistry.133:960-968.

35.Roos, Y. 2010. Glass transition temperature and its relevance in food processing. Annual review of food science and technology. 1:469-496.

36.He, Y. and Vardhanabhuti, B. 2021. Improved Heat Stability of Whey Protein Isolate by Glycation with Inulin. Dairy. 2:135-147.

37.Wang, Q., and Ismail, B. 2012. Effect of Maillard-induced glycosylation on the nutritional quality, solubility, thermal stability and molecular configuration of whey proteinv. International Dairy Journal. 25: 112-122.

38.Spotti, M.J., Martinez, M.J., Pilosof, A. M., Candioti, M., Rubiolo, A.C., and Carrara, C.R. 2014. Rheological properties of whey protein and dextran conjugates at different reaction times. Food Hydrocolloids. 38: 76-84.

39.Bier, J.M., Verbeek, C.J., and Lay, M.C. 2014. Thermal Transitions and Structural Relaxations in Protein‐B ased Thermoplastics. Macromolecular materials and engineering. 299:524-539.

40.Slade, L., and Levine, H. 1995. Water and the glass transition—dependence of the glass transition on composition and chemical structure: special implications for flour functionality in cookie baking. Journal of Food Engineering. 24: 431-509.

41.Ru, Q., Cho, Y. and Huang, Q. 2009. Biopolymer-stabilized emulsions on the basis of interactions between b-lactoglobulin and i-carrageenan. Frontiers of Chemical Science and Engineering in China. 3: 399-406.

42.Liu, J., Liu, W., Salt, L.J., Ridout, M.J., Ding, Y., and Wilde, P.J. 2018. Fish oil emulsions stabilized with caseinate glycated by dextran: Physicochemical stability and gastrointestinal fate. Journal of Agricultural and Food Chemistry. 67: 452-462.

43.O'Mahony, J.A., Drapala, K.P., Mulcahy, E.M., and Mulvihill, D.M. 2017. Controlled glycation of milk proteins and peptides: Functional properties. International Dairy Journal. 67:16-34.

44.Kato, A. 2002. Industrial applications of Millard-type protein– polysaccharide conjugates. Journal of Food Science and Technology. 8:193-199.

45.Wagner, J.R. and Gueguen, J. 1999. Surface functional properties of native, acid-treated, and reduced soy glycinin. Emulsifying properties. J. Agric. Food Chem. 47:2181–2187.

46.Plasencia, J., Pettersen, B. and Nydal, O.J. 2013. Pipe flow of waterin-crude oil emulsions: effective viscosity, inversion point and droplet size distribution. Journal of Petroleum Science and Engineering.101: 35- 43.

47.Mantzouridou, F., Spanou, A., and Kiosseoglou, V. 2012. An inulin-based dressing emulsion as a potential probiotic food carrier. Food Research International.46:260-269.

48.Ghosh, A.K., and Bandyopadhyay, P. 2012. Polysaccharide - protein interactions and their relevance in food colloids. The complex world of polysaccharides. 14:395-406.

49.McClements, D.J., and Jafari, S.M. 2018. Improving emulsion formation, stability and performance using mixed emulsifiers: A review. Advances in colloid and interface science. 251:55-79.